雙通道實(shí)時(shí)定量PCR 儀
【介紹】
DNA Engine Option 2 是一款雙波長(zhǎng)實(shí)時(shí)PCR 儀。96 個(gè)藍(lán)-綠 LED 順序激發(fā)樣品,通過(guò)二個(gè)光電二極管來(lái)檢測(cè)。兩個(gè)激發(fā)波長(zhǎng)范圍: 一為523–543nm(適用于FAM、SYBR Green I 等),還有一個(gè)是 540–700nm(適用于普遍使用的HEX、TEMRA、TET、VIC 等熒光素)。
DNA Engine Option 2 既可以支持普通的單重PCR,也可以支持多重 PCR。多重 PCR 除了可以節(jié)省試劑和時(shí)間外,還可以使內(nèi)對(duì)照的使用成為可能,從而對(duì)樣品PCR 效率有更進(jìn)一步的掌控。此外,多重PCR 也 使基因表達(dá)分析更為簡(jiǎn)便。其具體性能如下:
一次96 孔熒光檢測(cè)時(shí)間在3 秒之內(nèi),最大程度減少了熒光交叉
完美的溫度控制性能和梯度PCR 功能
Opticon Monitor 分析軟件可以非常簡(jiǎn)單地設(shè)置RT–PCR、多重PCR 等反應(yīng),以及DNA 定量、基因表達(dá)、等位基因等功能分析
LED 陣列與反應(yīng)板一一對(duì)應(yīng),保證激發(fā)光準(zhǔn)確進(jìn)入目標(biāo)管。發(fā)射光通過(guò)光電倍增管(PMTs)系統(tǒng)進(jìn)行信號(hào)放大。
【技術(shù)指標(biāo)】
Base thermal cycler |
DNA Engine |
Sample capacity |
96-well PCR plate (low-profile) or 12 x 0.2 ml 8-tube strips (low-profile) |
Sample volume |
10–100 µl (20 µl recommended) |
Dimensions (W x D x H) |
34 x 47 x |
Weight |
|
Fluorescence excitation range |
470–505 nm |
Fluorescence detection range |
Channel 1: 523–543 nm |
Linear dynamic range of starting copy number |
Up to 10 orders of magnitude |
Detection limit of template starting copy number |
Down to single initial template copy detection |
Gradient Specifications | |
Gradient range |
30– |
Temperature differential range |
1– |
Gradient accuracy |
± |
Temperature uniformity |
± |
Gradient calculator accuracy |
± |
Computer System Requirements (Computer NOT Included With System) | |
Model |
Desktop tower computer |
PCI card slot dimensions |
Full-size card, |
Operating system |
Windows XP Professional |
Storage capacity |
40 GB hard drive |
Memory |
512 MB RAM |
Port |
Communication (COM) |
Supplied With System | |
Data acquisition board |
National Instruments PCI-6036E, 200 x 10³ samples/sec |
Note: Specifications subject to change without notice. |